The aim of this study was to determine the effect of royal jelly on magnesium, calcium, sodium, potassium and chlorine levels in 1000 mg / day dose- smoking addicts with controlled experimental design. For this purpose, Fagerström Nicotine Dependence Scale was applied to smokers, a total of 20 adult male cigarette addicts (n = 10) with high addiction scores (6-7 points) were included in the study and the cigarette addict experimental group (n = 10) taking royal jelly supplementation. Criteria such as smoking and dependence of the individuals included in the study, not having any disease and not having allergies to honey derived foods were determined and groups were formed in this way. The experimental group was given liquid royal jelly (n = 10/1000 mg /day) supplemented in glass vials in the refrigerator at the same time every morning for three weeks. No application was made to the control group. In order to determine magnesium, calcium, sodium, potassium and chlorine levels in both groups, blood samples were analyzed one day before and one day after the study. SPSS 22.0 package program was used for statistical analysis of measured data of experimental and control groups. Paired Sample t-Test was used to compare the pre- and post-tests of the groups and Independent t-Test was used to compare the two groups. Statistical results were evaluated at p <0,05 significance level. In the analysis of the levels of magnesium, calcium, sodium, potassium and chlorine between the pre-test and post-test of the experimental group receiving royal jelly supplementation and the non-supplemented control group, no statistical significance was found (p> 0,05). In the intergroup analysis of the two groups, again no significant difference was found (p> 0,05). As a result of this study, it can be said that royal jelly supplementation at a dose of 1000 mg / day for 21 days does not affect magnesium, calcium, sodium, potassium and chlorine levels in cigarette addicts.